Journal of Food Processing & Technology

Chicken protein hydrolysates targeting mitochondria exert anti-atherosclerotic effect beyond plasma cholesterol-lowering activity in apoE-/- mice

21^st Euro-Global Summit on Food and Beverages

March 08-10, 2018 | Berlin, Germany

Thomas A Aloysius, Bodil Bjorndal, Anders Lund, Raza Slizyte, Pavol Bohov, Ana Karina Caravajal and Rolf K Berge

University of Bergen, Norway
SINTEF Sealab, Norway
Haukeland University Hospital, Norway

Scientific Tracks Abstracts: J Food Process Technol

Abstract:

Utilization of by-products from chicken: Protein hydrolysates of both marine and agricultural origin have many health benefits, including lipid lowering and anti-inflammatory effects. This study aimed to penetrate the effect of protein hydrolysates from chicken (CPHs) on obesity related disease and atherosclerosis. Firstly, a mouse obesity model was used to investigate the effect of four different CPH (CPH1-4) generated using specific hydrolyzation methods. C57BL/6 mice were fed a high-fat control diet (25% w/w casein) or CPHs diets (12.5% w/w CPH and 12.5% w/w casein) for 12 weeks, and plasma lipids, hepatic mitochondrial fatty acid oxidation and plasma cytokine levels was analysed. Diets containing CPH1-4 significantly reduced plasma levels of IL-1�?², IL-16, INF-�?³, TNF�?±, and MCP-1. In addition, diets containing CPH3 and 4 increased the hepatic mitochondrial fatty acid oxidation indicating a positive effect on fat burning. Diets with 12.5% w/w CPH3 and 4 were further studied in atherosclerosis-prone apoE-/- mice. After 12 weeks of high-fat feeding, CPH4 significantly reduced the atherosclerotic plaque area in the aortic arch compared to control (P<0.02), while CPH3 revealed reduced atherosclerosis at borderline significance ( P<0.09).The plasma level of cholesterol and triacylglycerol remained constant in the two experimental groups, but changed plasma fatty acid composition was observed resulting in an increased plasma level of C20:4n-3 and C20:5n-3 accompanied with increased delta-5 desaturase index and reduced plasma atherogenicity index (AI). Noteworthy, the AI correlated to the plague area. Moreover, the protein hydrolysate from CPH4 tended to decrease plasma levels of inflammatory mediators and a significantly reduced level of MCP-1 was found compared to control. Overall, these data show that protein hydrolysates from chicken, able to increase the mitochondrial fatty acid oxidation, can reduce atherosclerosis and attenuate systemic risk factors related to atherosclerotic disorders, but not directly related to changes in the level of plasma cholesterol. Recent publications 1. Wergedahl H, et al (2004) Fish protein hydrolysate reduces plasma total cholesterol, increases the proportion of HDL cholesterol and lowers acyl-CoA: cholesterol acyltransferase activity in liver of Zucker rats J. Nutr Jun. 134(6):1320-7. 2. Wergedahl H, et al (2009) Combination of fish oil and fish protein hydrolysate reduces the plasma cholesterol level with a concurrent increase in hepatic cholesterol level in high-fat-fed Wistar rats Nutrition Jan. 25(1):98â�?�?104. 3. Bj�?¸rndal B, et al (2013) A fish protein hydrolysate alters fatty acid composition in liver and adipose tissue and increases plasma carnitine levels in amouse model of chronic inflammation. Lipids Health Dis. 12:143. 4. Parolini C, et al (2014) A salmon protein hydrolysate exerts lipid-independent anti-atherosclerosis activity in ApoEdeficient mice. Plos One, 9(5):e97598. 5. Parolini C, et al (2017) Effect of dietary components from Antarctic krill on atherosclerosis in apoE-deficient mice. Mol Nutr Food Res. 61(12).

Biography :

Thomas A Aloysius has experience in Cancer Diagnostics, Tumor Molecular Biology and has worked with research on cancer molecular biomarkers for the past 20 years. The focus is now on nutrition, obesity, metabolic syndromes and dietary supplements to improve health, healthcare and wellbeing. The idea behind our research program is to bring new products, dietary supplements and food for special purposes to market.
Email:thomas.aloysius@uib.no