Journal of Blood Disorders & Transfusion

Antioxidant and anti-inflammatory properties of aerial parts of Heliotropium undulatum

Joint Event on Hematology, Immunology & Traditional Medicine

December 05-06, 2018 | Lisbon, Portugal

Menad Ahmed, Ameddah Souad, Deffa Ouafa, Aissaoui Hanane, Mekkiou Ratiba, Benayache Fadila and Benayache Samir

Universite des Freres Mentouri Constantine, Algerie

Posters & Accepted Abstracts: J Blood Disord Transfus

Abstract:

Background & Aim: Attenuation of the chronic inflammatory response and oxidative stress are a beneficial strategy to combat several human diseases. Algerian Heliotropium undulatum is considered of high importance due to its folk medicine used for the treatment of inflammatory disorders. The present study was undertaken to examine their antioxidant and anti-inflammatory properties using n-BuOH extracts of both of H. undulatum leaves (BEHUL) and stems (BEHUS). Methods: Both BEHUL and BEHUS were assessed for phytochemical screening (HPLC-TOF/MS analysis), antioxidant activity (in vitro model assay) and for anti-inflammatory activity (in vitro, in vivo model). Results: The obtained results revealed that both extracts (BEHUL) and (BEHUS) showed a significant reducing power in the following order: leaves>stems. Both fractions also exhibited a significant �OH. scavenging effect; this effect is more pronounced in the case of BEHUL as compared with BEHUS (IC50: 96 ?g/mL vs 150 ?g/mL). BEHUL exhibited a strong ability in iron chelation as evidenced by the efficiency in the suppressive of Fe2+-ferrozine complex formation. However BEHUS exhibited more anti-inflammatory effect in comparison to BEHUL which was evidenced by the NO radical scavenging activities. Inhibiting the hypotonicity induced haemolysis; inhibiting the heat induced albumin denaturation and the highest oedema inhibition. Conclusion: From the present study, it can be concluded that aerial parts of H. undulatum possess marked antioxidant and anti-inflammatory effects. These effects could possibly due to the different degree of polyphenols contents of both BEHUL and BEHUS fractions that may be clearly explained by the HPLC-TOF/MS analysis.

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