Mahendra Maharjan and Swapna Sah
Posters-Accepted Abstracts: J Bacteriol Parasitol
Enteric fever is an ancient disease but still it is an important public health problem in developing countries including Nepal.
A changing antibiotic susceptibility pattern of Salmonella Typhi and Salmonella Paratyphi A and emergence of multi
drug resistance has increased to a great concern globally. The present study was conducted to determine the antibiogram of
Salmonella serotype Typhi and Paratyphi isolated from patients suspected of enteric fever. A prospective study was carried out
from July 2012 to August 2012. The positive samples of Salmonella were collected from Alka Hospital by streaking in Nutrient
agar slant and transported aseptically to the Nepal Academy of Science and Technology (NAST) laboratory for the further
identification of strains and antibiotic susceptibility testing. Antibiotic susceptibility test was performed by modified Kirby-
Bauer disc diffusion method and results were interpreted by Clinical Laboratory Standards Institute (CLSI), 2012 guideline.
A total of 47 Salmonella serotype isolated from 896 blood culture samples revealed almost equal infection in male and female.
Age wise infection rate indicated higher among the age group less than 10 years. S. Typhi was isolated in 46.81% of cases while
S. Paratyphi A in 53.19% of cases. Both S. Typhi and S. Paratyphi A were found to be 100% susceptible to drugs amoxicillin,
chloramphenicol and ceftriaxone. Susceptibility to ofloxacin was 100% in S. Typhi and only 64% in S. Paratyphi A. Similarly
susceptibility of gentamicin, co-trimoxazole, ampicillin and nalidixic acid was 95.45%, 86.36%, 90.90% and 13.64% and 76%,
88%, 64% and 4% for S. Typhi and S. Paratyphi A respectively. Salmonella Paratyphi A was observed as a rapidly emerging
pathogen of enteric fever. There is increasing resistance of S. Typhi and S. Paratyphi A to many antibiotics and also there is reemergence
of chloramphenicol sensitivity in both strains of Salmonella.