Journal of Bacteriology & Parasitology

Amplification, cloning and expression of Brucella melitensis bp26 gene (OMP28) isolated from Markazi province (Iran) and purification of Bp26 protein

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Hosseini Seyed Davood1 and Azizpour M2

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Brucellosis is a debilitative disease that imposes costs on both economy and society. It is shown that although the vaccine
can prevent abortion, it does not provide complete protection against infection. In Iran, Brucella melitensis is a common
causative agent for brucellosis and BP26 protein of this bacterium having a good antigenesity and an important vaccine
candidate. In this study B. melitensis bp26 gene was cloned first in to PTZ57R/T vector and accessed on the PET28a vector
and sequenced. Recombinant vector transformed and expressed in to E. coli BL21 (DE3) and then recombinant protein was
purified with Ni-NTA column of chromatography against His tag. Obtained rOmp28 could be used as a research experimental
tool to find its potential as a detection kit and vaccine candidate.

Biography :

Hosseini Seyed Davood currently as Dean at Razi Vaccine & Serum Research Institute Central Area branch (Arak), Iran.