Ameliorative effect of ethanolic extract of Annona muricata against sodium arsenite: Induced hepatotoxicty in Wistar rats
Joint Event on 11th International Conference and Exhibition on Pharmacology and Ethnopharmacology & International Conference on Pharmaceutical Oncology
July 18-19, 2018 | Atlanta, USA

Adegboyega Adenike M, OA Odunola, KA Akinwumi, O Babalola and Akinwande B

University of Ibadan, Nigeria
The Polytechnic, Ibadan, Nigeria

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Ingestion of arsenic in drinking water causes cancer in multiple tissues and there is no cure. Cancer has become a great monster to the human race, as it places a significant emotional and economic burden on families and governments all over the world. For the management of arsenicosis, research is directed at chemoprevention using medicinally. In this study, we evaluated the in-vitro antioxidant and protection offered by Annona muricata L. (AM) against sodium arsenite-induced hepatotoxicity in rats. Antioxidant and radical scavenging activities of AM were compared to vitamin C and Butylated Hydroxytoluene (BHT). Proximate and phytochemical analyses were also carried out. Hepatoprotective study was investigated with six groups of rats that received distilled water (Control), 5.0 mg/kg bwt (body weight) of NaAsO2, 250 mg/kg bwt AM, 500 mg/kg bwt AM, NaAsO2 plus 250 mg/kg AM, NaAsO2 and 500 mg/kg AM. The NaAsO2 was given once on days 7, 14 and 21, while AM was administered orally daily for 21 days. Serum transaminases and alkaline phosphatase activities were determined and liver histopathology carried out. AM contained 2.00% ash, 1.94% crude fat, 25.65% crude fibre, 2.88% protein and 58.62% carbohydrate. Phytochemical analysis indicated the presence of alkaloids, flavonoids, and cardiac glycosides. The reducing power and metal chelating ability were in the order Vitamin C > BHT > AM, while for DPPH scavenging ability AM > Vitamin C > BHT. The NaAsO2 significantly (p < 0.05) increased the liver function enzymes relative to control. However, reduction of the marker enzymes and restoration of the severe vacuolation of hepatocytes in the NaAsO2 group to near normal is being markedly done with the help of AM treatment. Our findings suggest that AM may constitute a remedy against arsenic-induced hepatic injuries.