Manal Mohamed Kamel
Theodor Bilharz Research Institute, Egypt
Posters & Accepted Abstracts: J Nanomed Nanotechnol
Several hybidoma cell lines secreting monoclonal antibody against adult worm tegumental Schistosoma antigen were produced and preserved in liquid nitrogen. One MAb (6D/6F) was chosen for this study due to its high reactivity to schistosome antigens. Gold nanoparticles (AuNPs) were functionalized and conjugated with MAb . The study was conducted on serum samples of 116 subjects: 71 patients with S. mansoni eggs in their stools, 25 patients with other parasites and 20 negative healthy controls. Infected group were subdivided according to egg count in their stools into light infection, moderate and severe infection. Sandwich ELISA was performed using (AuNPs -MAb) for detection of circulating schistosomal antigen level (CSA) in serum samples of all groups and the results were compared with that after using conventional sandwich ELISA. Gold- MAb/ ELISA system could detect up to 10 ng/ml CSA compared to 85 ng/ml on using conventional ELISA and the optimal concentrations of AuNPs -MAb were found to be 12 folds less than that of MAb/ ELISA system. Sensitivity and specificity of sandwich ELISA using AuNPs-MAb were 100% & 97.8% compared to 87.3% & 93.38% respectively on using conventional ELISA system. The higher sensitivity and specificity of using AuNPs –MAb enabling detection of cases with light infection that were missed using conventional ELISA. Our data demonstrated that: Loading gold nanoparticles with MAb increases the sensitivity and specificity of sandwich ELISA for detection of CSA, thus active and light infections could be easily detected. This binding will decrease the amount of MAb consumed in the assay and lower the cost.
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