Journal of Liver

A proteomic approach for the involvement of the ANXA4 in HCC: Moving towards an understanding of tumor progression

2^nd International Conference on Hepatology

May 09-11, 2016 Chicago, USA

Nikhat Ahmed Siddiqui, Shamim Mushtaq, Been Hasan, Ayesha Khan, Meraj Zehra, Ayesha Siddiqui, Sobia Manzoor and M A Rahman

Ziauddin University, Pakistan
University of Karachi, Pakistan

Posters & Accepted Abstracts: J Liver

Abstract:

Proteomics-based clinical studies have been shown to be promising strategies for the discovery of novel biomarkers of a particular disease. To gain insight into development of Hepatocellular carcinoma utilizing promising strategies (2 dimensional electrophoresis and ESI-QTOF-MS/MS) we aimed to identify potential biomarkers for hepatocellular carcinoma (HCC) and analyzed a set of 115 samples (HCC=50, Fibrosis= 50 & Control= 15). From the series of seventeen differentially expressed proteins, collectively we identify annexin A4 (ANXA4) an intracellular Ca2+ sensor, as a new biomarker for early diagnostic and prognostic potential. Expression of ANXA4 was found to be up- regulated (fold change �?� + 2.0, P �?�0.05) in HCC as compared to fibrosis and control. After validated current finding, we applied in silico analysis to integrate the data generated from proteomics technologies. We extend this current understanding to demonstrate the significantly induced phosphorylation and S-nitrosylation signals by insilico study, suggesting a role of ANXA4 in cell survival may have implications for cancer progression and chemoresistance. Moreover, we revealed interacting partner of ANXA4 bestowed with critical capabilities, namely apoptosis, cell cycling, anticoagulation, cell motility and stress resistance that together demonstrate their possible role in cancer progression. Overall, our results shed new light on the potential of biomarker ANXA4 as biomarker used for early diagnosis, prognosis prediction, and personalized treatment of HCC.

Biography :

Email: nikhat_ahmed14@yahoo.co.uk