Manan AAA

Department of Viral Vaccines Production, Central Veterinary Research Laboratory (CVRL), Animal Resources Research Corporation (ARRC), P.O. Box 8067, El Amarat, Khartoum, Sudan

Publications
  • Original Research Article   
    Development of Inactivated Newcastle Disease Vaccine in Sudan
    Author(s): Manan AAA*, Kheir MAS, Ballal A, Nour TAM, Wegdan H. Ali and Fatima AT

    Using 1-2 and La-Sota strains two inactivated Newcastle Disease (ND) vaccines were produced. The vaccine viruses were inactivated by treatment with 0.05% laboratory-grade formaldehyde, then each inactivated vaccine was preparedas water in oil (W/O) emulsion. For each emulsion, the aqueous phase ratio was (2.4:1) (allantoic fluid: tween 80) respectively. While the oil phase contains (9:1) (paraffin oil: Manidmonoleate (span 80)) as an oil emulsifier. The prepared vaccines were subjected to physical tests including stability, viscosity, and quality of emulsification completeness. The two vaccines were confirmed to be sterile, stable for 30 days at 37?C, and for 6 months at 4 co. the viscosity was 4 ml/8 second. Tests for safety, immunogenicity, and efficacy (challenge test) as well as cross-protection evidence for the two vaccines was performed in -one day- old broiler chicks. For phase.. View more»

    Abstract PDF