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Le Van Chuong
HCMC University Medical Center, Vietnam
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Research
Development and Testing of Trial Samples for Specific Serodiagnosis of Fasciola gigantica via External Quality Assessment
Author(s): Vu Quang Huy*, Tran Thien Trung, Ha Manh Tuan, Vu Tri Thanh, Le Van Chuong, Nguyen Lam Duc Vu, Huynh Thi Diem Phuc, Bui Quang Sang and Van Hy Triet
Objective: To build a procedure for production of samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via an external quality assessment scheme, and to evaluate the homogeneity and stability of the trial samples. Methods: In this experimental study, samples contained an anti-Fasciola gigantica antibody according to screening by ELISA, followed by confirmation by Western blotting were collected. All samples were tested and found negative for other helminth, especially of trematode antibodies. The samples were also negative for anti-HIV-1 and -2 antibodies, anti-HCV antibodies, and HBs antigen. The samples were prepared by freeze-drying and freezing methods, the stability and homogeneity were evaluated each 2, 4, 8, 12 and up to 24 weeks. Results: We produced three lots of serum.. View more»