Journal of Carcinogenesis & Mutagenesis

Journal of Carcinogenesis & Mutagenesis
Open Access

ISSN: 2157-2518

Keiji Kuwabara

Division of Applied Life Science, Graduate School of Engineering, Sojo University, Kumamoto, Japan

  • Research Article   
    Inhibitory Effect of Hybrid Liposomes on the Growth of NP2 Glioma Cells
    Author(s): Yoko Matsumoto*, Keiji Kuwabara and Hideaki Ichihara

    The inhibitory activity of hybrid liposomes (HLs), composed of 90 mol% L-α-dimyristoyl-phosphatidylcholine (DMPC) and 10 mol% polyoxyethylene (25) dodecyl ether (C12(EO)25), was investigated in human glioma NP2 cells. HLs with a hydrodynamic diameter below 100 nm persisted for 4 weeks. The inhibitory effect of HLs on the proliferation of NP2 cells was evaluated. Induction of apoptosis in NP2 cells treated with HL was measured through a PI assay and the TUNEL method. HLs caused apoptosis in NP2 cells through the mitochondrial pathway. An increase of AIF protein expression was observed in HL-treated cells. Cellular membrane fluidity of NP2 cells was also increased as revealed by the fluorescence depolarization method. Enhanced HL accumulation in the membrane of NP2 cells was observed... Read More»

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