Journal of Membrane Science & Technology

Journal of Membrane Science & Technology
Open Access

ISSN: 2155-9589

Bozena Michniak-Kohn

Center for Dermal Research and Department of Pharmaceutical Sciences, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA

  • Research Article   
    Camera-less Terahertz Imaging Investigation of the Interaction of Factor XII Protein with the Human Stratum Corneum
    Author(s): Zia Shariat-Madar, Aunik Rahman, Bozena Michniak-Kohn, Guacyara Da Motta and Anis Rahman*

    This paper reports the use of camera-less terahertz imaging technique to measure zymogen factor XII (FXII) conformational changes in solution and upon binding to stratum corneum in the absence of its known endogenous potentiating complex, high molecular weight kininogen (HK)-Prekallikrein (PK) in real-time. FXII is synthesized in the liver and secreted into the circulation to serve as a blood coagulation factor. FXII has diverse biological functions. First, it serves as an accessary molecule to extend the generation of thrombin-induced platelet adhesion to the initially formed platelet monolayer. Secondly, FXII provides a robust stimulus for Bradykinin (BK)-induced hyper-permeability by disrupting vascular barriers. Using in vitro assays, studies have shown that FXII activation can occur on negatively charged surfaces. This activation is accelerated in the presence of kallikrein or ac.. Read More»

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