Journal of Data Mining in Genomics & Proteomics

Yongjun Wen

Publications

• Research Article
  Immunoproteomic Analysis of Chinese Brucella Vaccine Strain M5 and New Immunogenic Proteins
  Author(s): Yanling Yang, Jigang Yin, Xinglong Wang, XiuranWang, Fengxue Wang, Yongjun Wen, Hailong Qu and JiankeWangYanling Yang, Jigang Yin, Xinglong Wang, XiuranWang, Fengxue Wang, Yongjun Wen, Hailong Qu and JiankeWang
  
  Brucellae are facultative intracellular gram-negative bacteria that cause human disease and significant worldwide economic loss due to infection of livestock. Available vaccines against Brucella spp. are live attenuated Brucella strains. In order to engineer a better vaccine to be used in animals and humans, our laboratory aims to develop an innocuous subunit vaccine. Particularly, we are interested in Brucella membrane proteins (MPs). In this study, an immunoproteomic approach was utilized to identify novel antigenic candidate proteins from Chinese Brucella vaccine strain M5 membrane proteins. The membrane proteins were separated by 2-DE and analyzed by western-blotting for their reactivity with the antiserum obtained from goats naturally infected by Brucella. Of a total of 9 immunogenic proteins identified, 7 were shown to be the novel antigens for Brucella spp. Some of the major pr.. View More»
  DOI: 10.4172/2153-0602.1000117

  Abstract PDF