Journal of Microbial & Biochemical Technology

Yanhong Liu

Publications

• Research Article
  Construction of Listeria monocytogenes Mutants with In-Frame Deletions in Putative ATP-Binding Cassette (ABC) Transporters and Analysis of Their Growth under Stress Conditions
  Author(s): Yanhong Liu, Marina Ceruso, Nereus W. Gunther IV, Tiziana Pepe, Maria Luisa Cortesi and Pina FratamicoYanhong Liu, Marina Ceruso, Nereus W. Gunther IV, Tiziana Pepe, Maria Luisa Cortesi and Pina Fratamico
  
  Listeria monocytogenes is a food-borne pathogen that is difficult to eliminate since it can survive under different stress conditions such as low pH and high salt. Understanding its survival under stress conditions is important in controlling this pathogen in food. ABC transporters have been shown to be induced in L. monocytogenes subjected to high pressure and nisin treatments; therefore, we hypothesized that genes encoding the ABC transporters may be involved in general stress responses. To study the function of these genes, deletion mutants of ABC transporter genes (LMOf2365_1875, LMOf2365_1877) were created in L. monocytogenes F2365, and these deletion mutants were tested under different stress conditions. Compared to the wild type, ΔLMOf2365_1875 and ΔLMOf2365_1877 showed slower growth under nisin (250 μg/ml) and acid (pH 5) treatm.. View More»
  DOI: 10.4172/1948-5948.1000085

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