Journal of Diabetes & Metabolism

ISSN - 2155-6156

Yang Xiao

Yang Xiao

China

Publications
  • Research Article
    Obese Patient-Derived Sera Have Proinflammatory Effect
    Author(s): Lan Yao, Kuan Yan, Yang Xiao, Aimin Xu, Zhiguang Zhou and Shiping LiuLan Yao, Kuan Yan, Yang Xiao, Aimin Xu, Zhiguang Zhou and Shiping Liu

    Aim: Chronic inflammation of adipose tissues plays a key role in obesity-induced metabolic disorders. We examined effects of sera from obese patients with or without different compositions of metabolic disorders on the activation of Toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signaling pathway in the human monocytic leukemia cell line (THP-1), which have never been reported. Methods: With or without pretreatment of TLR4 monoclonal antibody, THP-1 cells were incubated for 48h by sera from 45 obese patients with or without metabolic disorders and 15 controls. The level of TLR4 in THP-1 cells, intracellular level of phosphorylated NF-κB p65, IL-1β and TNF-α levels in cell culture supernatants were measured. Results: Compared with controls, the expression level of TLR4, NF-κB p65, IL-1β and TNF-α were significantly.. View More»

    DOI: 10.4172/2155-6156.1000604

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