Biochemistry & Analytical Biochemistry

William V Holt

Publications

• Research Article
  Seminal Plasma Proteins Prevent Detrimental Effects of Ram Sperm Cryopreservation and Enhance the Protective Effect of Lecithin
  
  Author(s): SIgnacio Del-Valle, Adriana Casao, Rosaura Pérez-Pé, William V Holt, José A Cebrián-Pérez and Teresa Muiño-BlancoSIgnacio Del-Valle, Adriana Casao, Rosaura Pérez-Pé, William V Holt, José A Cebrián-Pérez and Teresa Muiño-Blanco
  
  Background: One of the main drawbacks of refrigerated and frozen-thawed semen is lipid peroxidation and the subsequent generation of reactive oxygen species from cellular metabolism. In this study, we tested the hypothesis that the use of antioxidant compounds improves the frozen-thawed sperm quality. We analysed the effect of different antioxidants, or antioxidant-like additives, upon the processes involved in cooling and freeze-thawing ram semen. Methods: Sperm motility, plasma membrane integrity and stability, and mitochondrial membrane potential (MMP) were tested immediately after thawing and following incubation for 3 h and 6 h at 37°C. Results: The addition of oleic/linoleic acid did not improve sperm viability, although it resulted in increased motility after refrigeration and rewarming, similar to that found with pyruvic acid. In frozen-thawed samples, the effect of 75 mM .. View More»
  DOI: 10.4172/2161-1009.1000319

  Abstract