Modern Chemistry & Applications

Wang L

Publications

• Research Article
  Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection
  Author(s): Wang L and Lin BWang L and Lin B
  
  A novel method for the determination of isopropyl-β-D-thiogalactopyranoside (IPTG), a sulfur-containing compound, a pharmaceutical additive in commercial recombinant human growth hormone (rhGH), has been developed using pulsed electrochemical detection (PED), following ion Chromatographic separation. A Dionex-500 ion chromatograph coupled with an electrochemical detector was employed, equipped with a gold working electrode and an Acclaim 300 analytical C18 column, with a mobile phase consisting of sodium acetate (NaOAc) buffer (pH 5.45, 0.01 mol/L) and acetonitrile (ACN)(90/10, v/v). Upon optimization, IPTG was found to have a limit of detection of 1 ng/mL (0.1 μmol) with 25 uL injection volume. This method was successfully applied to the determination of IPTG in rhGH samples with the characteristics of simplicity, high sensitivity and good repeatability. .. View More»
  DOI: 10.4172/2329-6798.1000232

  Abstract PDF