Journal of Clinical & Experimental Pharmacology

Wahajul Haq

Publications

• Research Article
  BTF3-Promoter Based Screening of Anti- Human Breast Cancer Compounds
  Author(s): Kavita Rawat, Wahajul Haq and Raj Kamal TripathiKavita Rawat, Wahajul Haq and Raj Kamal Tripathi
  
  Basic Transcription Factor 3 (BTF3) is a Transcription factor known to differentially express in different forms of cancer; where in gastric cancer, silencing of BTF3 induced apoptosis. Previously, we reported Human β casein fragment 54-59 (NS) down modulates BTF3 expression in Human THP-1 cells. In the present study, we developed an in vitro model targeting BTF3 Promoter for screening of compounds down regulating BTF3 and to study the impact of BTF3 down regulation on apoptosis in MCF-7 cell line. We further confirmed the efficacy of NS to down regulate BTF3 in MCF-7 cells through western blotting. NS and its analogs AN1, AN2 and AN3 were screened on BTF3 promoterreporter construct during transient transfection in MCF-7 cell line, where the expression of reporter was found to be down regulated upon treatment. This study was further confirmed by immunoblotting which produced simi.. View More»
  DOI: 10.4172/2161-1459.1000183

  Abstract PDF