Biochemistry & Analytical Biochemistry

Valeria Polzonetti

Publications

• Research Article
  A Proteomic Study on Donkey Milk
  Author(s): Silvia Vincenzetti, Adolfo Amici, Stefania Pucciarelli, Alberto Vita, Daniela Micozzi, Francesco M Carpi, Valeria Polzonetti, Paolo Natalini and Paolo PolidoriSilvia Vincenzetti, Adolfo Amici, Stefania Pucciarelli, Alberto Vita, Daniela Micozzi, Francesco M Carpi, Valeria Polzonetti, Paolo Natalini and Paolo Polidori
  
  In children with Cow Milk Protein Allergy (CMPA), when it is not possible to breast feed or to use cow milk, the clinical use of donkey milk is considered since several studies have demonstrated the high similarity of donkey milk compared to human milk. An analysis was performed on donkey milk protein profile by two-dimensional electrophoresis (2-DE) followed by N-terminal sequencing in order to give a panoramic view of the proteins that are present in donkey milk. Furthermore, the interest was focused on the casein fractions and on their phosphorylation degree that may influence the calcium binding ability of caseins. At this purpose experiments on donkey milk casein dephosphorylation have been performed and the dephosphorylated casein fractions have been identified after 2-DE analysis followed by N-terminal sequencing. Among caseins were found mainly &al.. View More»
  DOI: 10.4172/2161-1009.1000109

  Abstract PDF