Journal of Neonatal Biology

Thaddeus G Golos

Publications

• Research Article
  Listeria monocytogenes Infection Reduces the Functionality of Human Choriocarcinoma JEG-3 Cells
  Author(s): Keith P Poulsen, Nancy G Faith, Thaddeus G Golos, Maria Giakoumopoulos and Charles J CzuprynskiKeith P Poulsen, Nancy G Faith, Thaddeus G Golos, Maria Giakoumopoulos and Charles J Czuprynski
  
  Listeria monocytogenes is a facultative intracellular bacterium associated with foodborne disease outbreaks. Pregnant women, and their fetuses, are at particular risk for adverse outcomes to pregnancy. The mechanisms by which L. monocytogenes infection disrupts homeostasis in the placenta are incompletely understood. In this study, we tested the ability of L. monocytogenes to invade, multiply within, and alter the function of JEG-3 cell monolayers. The effects of L. monocytogenes infection on JEG-3 cell monolayer integrity, cell function, cell metabolic activity, and cell death were measured with Transepithelial Electrical Resistance (TEER), Alamar Blue reduction, and LDH release, respectively. L. monocytogenes readily infected JEG-3 cells and multiplied to a peak number of intracellular organisms at 10 hours post-infection. L. monocytogenes infection decreased TEER of the JEG-3 monol.. View More»
  DOI: 10.4172/2167-0897.1000125

  Abstract PDF