Sydney Benchetrit
Israel
Research Article
Vitamin D and Vascular Smooth Muscle Cells: Gene Modulation Following Exposure to a Diabetic-Like Environment
Author(s): Tali Zitman-Gal, Janice Green, Metsada Pasmanik-Chor, Varda Oron-Karni, Jacques Bernheim and Sydney BenchetritTali Zitman-Gal, Janice Green, Metsada Pasmanik-Chor, Varda Oron-Karni, Jacques Bernheim and Sydney Benchetrit
Background: Diabetes mellitus (DM) is one of the leading causes of chronic vascular disease, which can accelerate the development of cardiovascular and renal disorders. Vascular smooth muscle cells (VSMC) are involved in the occurrence of vascular atherosclerosis and arteriosclerosis. Calcitriol might inhibit endothelial proliferation, blunt angiogenesis, and serve as a cardioprotective agent. We evaluated the impact of a diabetic-like environment and calcitriol on VSMC gene and protein expression. Methods: VSMC were treated for 24 hours using 200 μg/ml AGE-HSA, 250 mg/dl glucose and 10-9 or 10-10 mol/l calcitriol. Microarray gene chip analysis, real time PCR, western blot and ELISA techniques were used to determine gene and protein expression. Results: A total of 2,693 genes were differentially expressed in a diabetic-like environment compared to control, with 1796 up-regulated an.. View More»
