Suman B Sharma
University College of Medical Sciences (University of Delhi) and G.T.B. Hospital,
Dilshad Garden, Delhi-110095
India
Research Article
Study on Association of APOB Gene Polymorphism with Glycation of Low Density Lipoproteinin Type 2 Diabetes
Author(s): Kapil Dev, Seema Garg, Suman B Sharma, Amitesh Aggarwal and Madhu SVKapil Dev, Seema Garg, Suman B Sharma, Amitesh Aggarwal and Madhu SV
Objectives: Sustained hyperglycemia results in non-enzymatic glycation of Apo B or LDL particle which may affect its recognition and uptake. Increase in circulating LDL levels is a vital contributor for atherosclerosis. APOB gene (c.12669 G>A, p.GLN4154LYS) polymorphism is believed to be associated with coronary artery disease. We thus designed our study to evaluate association of APOB gene polymorphism with Apo B glycation in type 2 diabetic patients. Methods: A total of 45 non diabetic controls and 45 type 2 diabetic patients participated in this study. Following an overnight fast, venous blood was collected and analyzed for glycemic status, lipid profile and other biochemical parameters. Apo B was estimated using nephelometry, Glycated LDL was estimated by ELISA. PCR-RFLP was used to determine the DNA polymorphism in the APOB gene using EcoR1. Results: Polymorph.. View More»
