Clinical Microbiology: Open Access

Sinem Oktem-Okullu

Publications

• Research Article
  A Multiplex-Urease PCR Assay for Detection of Helicobacter pylori Infection Directly from Gastric Biopsy Specimens and Comparison of Multiplex- Urease PCR Results with Rapid Urease Test and Histopathology
  Author(s): Sinem Oktem-Okullu, Tanıl Kocagöz, Arzu Tiftikçi, Ayca Sayi-Yazgan, Nurdan Tozun, Murat Saruc, Bahattin Cicek, Eser Vardareli, Ugur Sezerman and Ahmet-Sinan YavuzSinem Oktem-Okullu, Tanıl Kocagöz, Arzu Tiftikçi, Ayca Sayi-Yazgan, Nurdan Tozun, Murat Saruc, Bahattin Cicek, Eser Vardareli, Ugur Sezerman and Ahmet-Sinan Yavuz
  
  Purpose: To develop a multiplex-urease PCR assay specific for ureA and ureB genes directly from gastric biopsy specimens and compare the results of multiplex-urease PCR assay with rapid urease test (RUT) and histopathology. Methodology: This study was conducted on 109 patients. A RUT was run; histopathological staining and multiplex urease PCR were applied to biopsy specimen to detect H. pylori. Multiplex-urease PCR, RUT and histopathology results were compared by calculating Cohen’s kappa coefficient. Results: A multiplex-urease PCR assay specific for ureA and ureB genes was developed to detect H. pylori directly from biopsy specimens. Cohen's kappa coefficient results of histopathological staining and multiplex-urease PCR indicate a substantial agreement. There was a moderate agreement between the resul.. View More»
  DOI: 10.4172/2327-5073.1000279

  Abstract PDF