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Shuye Wang
Shuye Wang
The First Affiliated Hospital Harbin Medical University,
23 Youzheng Street Nan Gang District, 150001 Harbin
China
Publications
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Research Article
Detection of the Impact of Bisphosphonate on Multiple Myeloma using Lactadherin
Author(s): Shuye Wang, Xiaomin Zhang, Jinxiao Hou, Yafeng Chen and Yueyue FuShuye Wang, Xiaomin Zhang, Jinxiao Hou, Yafeng Chen and Yueyue Fu
Background: Explore the impact of second-generation bisphosphonate drugs pamidronate on proliferation and apoptosis of human multiple myeloma cell line in vitro. Verify the synergistic effect of combination of pamidronate and melphalan on apoptosis of myeloma cells and demonstrate that lactadherin is the most effective probe for apoptosis detection. Method: (1) Myeloma cells were treated with pamidronate or combination of melphalan and pamidronate at different concentrations. The cells were divided into 12 groups and treated with the drugs at intervals of 24 hours for 48 hours. After 48 hours treatment, inhibition rate of cell growth in different group were measured by MTT. (2) Two groups were treated with PBS buffer. PS exposure of the cells treated with drugs was detected using FITC-Annexin-V and FITC-lactadherin by flow cytometry. .. View More»
DOI: 10.4172/2155-9864.1000281