Biochemistry & Analytical Biochemistry

Shin-ichiro Umemura

Publications

• Research Article
  Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis by 4-Formyloximeetylidene-3-Hydroxyl-2-Vinyl-euterio-Porphynyl (IX)-6-7-Diaspartic Acid (ATX-S10)
  Author(s): Nagahiko Yumita, Yumiko Iwase, Koji Nishi, Toshihiko Ikeda, Toshio Fukai, Kazuyoshi Takeda, Kenji Onodera, Shin-ichiro Umemura, Kazuho Okudaira and Yasunori MomoseNagahiko Yumita, Yumiko Iwase, Koji Nishi, Toshihiko Ikeda, Toshio Fukai, Kazuyoshi Takeda, Kenji Onodera, Shin-ichiro Umemura, Kazuho Okudaira and Yasunori Momose
  
  Background: In this study, we investigated the induction of apoptosis by ultrasound in the presence of the photochemically active chlorine, 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-porphynyl (IX)-6-7-diaspartic acid (ATX-S10). Methods: HL-60 cells were exposed to ultrasound for up to 3 min in the presence and absence of ATX-S10, and the induction of apoptosis was examined by analyzing cell morphology, DNA fragmentation, and caspase-3 activity. Results: Cells treated with 80μM ATX-S10 and ultrasound clearly showed membrane blebbing and cell shrinkage, whereas significant morphologic changes were not observed in cells exposed to either ultrasound or ATX-S10 alone. Also, DNA ladder formation and caspase-3 activation were observed in cells treated with both ultrasound and ATX-S10 but not in cells treated with ultrasound or ATX-S10 alone. In addition, the .. View More»
  DOI: 10.4172/2161-1009.S2-001

  Abstract PDF