Biochemistry & Analytical Biochemistry

Shilpa Gupta

Publications

• Research Article
  Variability for Leaf and Seed Glucosinolate Contents and Profiles in a Germplasm Collection of the Brassica juncea
  Author(s): Shilpa Gupta, Manjeet K Sangha, Gurpreet Kaur, Amarjeet K Atwal, Shashi Banga and Surinder S. BangaShilpa Gupta, Manjeet K Sangha, Gurpreet Kaur, Amarjeet K Atwal, Shashi Banga and Surinder S. Banga
  
  Aim: A renewed interest in glucosinolates (GSLs) as compounds with biocidal and anticarcinogenic activity demands evaluation of the available variability in germplasm collections. The objective of the present study was to evaluate a germplasm collection of the Brassica juncea for total content and profile of leaf and seed GSLs. Methods: A total of 366 entries of a RIL population derived from cross of NUDH-YJ-04 and RL-1359, were nondestructively analyzed by near-infrared reflectance spectroscopy by means of previously developed calibration equations. Out of 366 lines, 97 lines were selected on the basis of glucosinolate range and further analyzed by ultra performance liquid chromatography for total GSL content and the concentrations of individual components i.e. sinigrin, glucoiberin, epiprogoitrin, gluconapin, gluconasturtiin and gluconeobrassicin. Resul.. View More»
  DOI: 10.4172/2161-1009.1000120

  Abstract PDF