Sandy-Belle Tabbouny
Lebanon
Research Article
Oxidative Stress Levels in Buccal Cells Using MAWI Collection Tubes
Author(s): Sandy-Belle Tabbouny, Elinora Chamoun, Nataliia Pavliuchenko and Marcel BassilSandy-Belle Tabbouny, Elinora Chamoun, Nataliia Pavliuchenko and Marcel Bassil
Background: Oxidative Stress (OS) is defined as an imbalance between oxidants and antioxidants, in favor of oxidants, potentially leading to DNA damage. Benzo[a] pyrene (B(a)P), a representative DNA-damaging mutagenic/ carcinogenic Polycyclic Aromatic Hydrocarbons (PAH), can lead to the final mutagen Benzo(a)Pyrene Diol Epoxide (BPDE).
Methods: The extent of oxidative DNA damage is investigated in population studies using easily obtained cells. Buccal cell usage has been shown by many to be a cost effective, non-invasive and safe method to isolate DNA for various biological experiments. In this experimental research of 40 participants, equally divided between industry and academia, we compared the DNA concentration, purity, and associated levels of BPDE-DNA damage. Buccal cells were collected using ISWAB-DNA tubes, and DNA was then extracted.. View More»
DOI:
10.4172/2329-6887.1000225