Journal of Data Mining in Genomics & Proteomics

Robert Stephens

Publications

• Research Article
  A Benchmark Study on Error Assessment and Quality Control of CCS Reads Derived from the PacBio RS
  Author(s): Xiaoli Jiao, Xin Zheng, Liang Ma, Geetha Kutty, Emile Gogineni, Qiang Sun, Brad T. Sherman, Xiaojun Hu, Kristine Jones, Castle Raley, Bao Tran, David J. Munroe, Robert Stephens, Dun Liang, Tomozumi Imamichi, Joseph A. Kovacs, Richard A. Lempicki and Da Wei HuangXiaoli Jiao, Xin Zheng, Liang Ma, Geetha Kutty, Emile Gogineni, Qiang Sun, Brad T. Sherman, Xiaojun Hu, Kristine Jones, Castle Raley, Bao Tran, David J. Munroe, Robert Stephens, Dun Liang, Tomozumi Imamichi, Joseph A. Kovacs, Richard A. Lempicki and Da Wei Huang
  
  PacBio RS, a newly emerging third-generation DNA sequencing platform, is based on a real-time, single-molecule, nano-nitch sequencing technology that can generate very long reads (up to 20-kb) in contrast to the shorter reads produced by the first and second generation sequencing technologies. As a new platform, it is important to assess the sequencing error rate, as well as the quality control (QC) parameters associated with the PacBio sequence data. In this study, a mixture of 10 prior known, closely related DNA amplicons were sequenced using the PacBio RS sequencing platform. After aligning Circular Consensus Sequence (CCS) reads derived from the above sequencing experiment to the known reference sequences, we found that the median error rate was 2.5% without read QC, and improved to 1.3% with an SVM based multi-parameter QC method. In addition, a De Novo assembly was used as a dow.. View More»
  DOI: 10.4172/2153-0602.1000136

  Abstract PDF