Journal of Stem Cell Research & Therapy

Riitta Suuronen

Publications

• Research Article
  Growth and Differentiation of Human Dental Pulp Stem Cells Maintained in Fetal Bovine Serum, Human Serum and Serum-free/Xeno-free Culture Media
  Author(s): Rashi Khanna-Jain, George K.B. Sandor, Riitta Suuronen, Bettina Mannerstrom, Susanna Miettinen, Sari Vanhatupa and Annukka VuorinenRashi Khanna-Jain, George K.B. Sandor, Riitta Suuronen, Bettina Mannerstrom, Susanna Miettinen, Sari Vanhatupa and Annukka Vuorinen
  
  Introduction: Dental pulp stem cells (DPSCs) are an accessible cell source with therapeutic applicability in regeneration of damaged tissues. Current techniques for expansion of DPSCs require the use of Fetal Bovine Serum (FBS). However, animal-derived reagents stage safety issues in clinical therapy. By expanding DPSCs in serumfree/ xenofree medium (SF/XF-M) or in medium containing human serum (HS-M), the problems can be eliminated. Therefore, the aim of our study was to identify suitable cell culture media alternatives for DPSCs. Methods: We studied the isolation, proliferation, morphology, cell surface markers (CD29, CD44, CD90, CD105, CD31, CD45 and CD146), stemness markers expression (Oct3/4, Sox2, Nanog and SSEA-4) and in vitro multilineage differentiation of DPSCs in HS-M or SF/XF-M in comparison to FBS-M... View More»
  DOI: 10.4172/2157-7633.1000126

  Abstract PDF