Journal of Data Mining in Genomics & Proteomics

RH Brlansky

Publications

• Review Article
  A Case Study on Discovery of Novel Citrus Leprosis Virus Cytoplasmic Type 2 Utilizing Small RNA Libraries by Next Generation Sequencing and Bioinformatic Analyses
  Author(s): Avijit Roy, Jonathan Shao, John S Hartung, William Schneider and RH BrlanskyAvijit Roy, Jonathan Shao, John S Hartung, William Schneider and RH Brlansky
  
  The advent of innovative sequencing technology referred to as “Next-Generation” Sequencing (NGS), provides a new approach to identify the ‘unknown known’ and ‘unknown unknown’ viral pathogens without a priori knowledge. The genomes of plant viruses can be rapidly determined even when occurring at extremely low titers in the infected host. The method is based on massively parallel sequencing of the population of small RNA molecules 18-35 nucleotides in length produced by RNA silencing host defense. Improvements in chemistries, bioinformatic tools and advances in engineering has reduced the costs of NGS, increased its accessibility, and enabled its application in the field of plant virology. In this review, we discuss the utilization of the Illumina GA IIX platform combined with the application of molecular biology and bioinformatic tools for the disc.. View More»
  DOI: 10.4172/2153-0602.1000129

  Abstract PDF