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Reiko Ikeda
Reiko Ikeda
Japan
Publications
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Research Article
Peptidoglycan Synergistically Augments Production of Allergic Mediators from Murine Mast Cells in Combination with Muramyldipeptide
Author(s): Katsuhiko Matsui, Keisuke Shigehara and Reiko IkedaKatsuhiko Matsui, Keisuke Shigehara and Reiko Ikeda
Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by superficial Staphylococcus aureus colonization and an increased number of mast cells in the lesional skin, the immunopathologic features varying according to lesion duration. Objective: The present study was conducted to clarify the effects of S. aureus cell wall components on production of allergic mediators from murine mast cells. Methods: Peptidoglycan (PEG) and/or muramyldipeptide (MDP) were/was used to stimulate murine mast cells, and the resulting culture supernatants were assayed for Th1 and Th2 chemokines and histamine release. Chemokine production was assessed using reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Histamine release was measured using a comp.. View More»
DOI: 10.4172/2155-6121.1000256