Journal of Bacteriology & Parasitology

Rahmehr Shafiei

Publications

• Research Article
  Molecular Survey of Theileria annulata in Cattle by PCR - RFLP Method in Iran
  Author(s): Jamal Akbari, Javad Javanbakht, Moussa Tavassouli, Mohammad Tabatabai and Rahmehr ShafieiJamal Akbari, Javad Javanbakht, Moussa Tavassouli, Mohammad Tabatabai and Rahmehr Shafiei
  
  Theileriosis is one of the infectious diseases of cattle and buffalo in tropical countries and Mediterranean region that is of a considerable economical value, generated by Theileria haemoprotozoan and transferred by vetors belonging to Ixodidea. The present study investigates 52 samples in west and east Azerbaijan, Sanandaj and Kermanshah in Iran (2009). In this study, Theileria annulata was separated from ill and healthy cattle by PCR-RFLP method. Especial primers were achieved from SmI-2 gene for this procedure. A fragment of 270bp was separated from the gathered blood samples. PCR products were distinguished by agarose gel and ultraviolet rays system. Among 22 naturally infected cattle, 13 cases (59.09%) were positive in blood smear and 18 (81.81%) were positive in PCR. Among the random sampled healthy cattle, from 30 cases, 4 cases (13.32%) were positive in blood smear a.. View More»
  DOI: 10.4172/2155-9597.1000135

  Abstract PDF