Prasanth Kumar M
Vignan Pharmacy College,
Vadlamudi, Guntur, Andhra Pradesh
India
Research Article
Inhibitory Effects of Oncoba Spinosa on Key Enzymes Related to Diabetes Mellitus (α-Amylase and α-Glucosidase) and Obesity (Pancreatic Lipase) in Vitro
Author(s): Prasanth Kumar M, Suba V, Rami Reddy B and Srinivasa Babu PPrasanth Kumar M, Suba V, Rami Reddy B and Srinivasa Babu P
Aim and Objective: Oncoba spinosa Forssk. (Flacourtiaceae) (OS) is traditionally used for the management of diabetes mellitus. In previous studies, we studied the effect of OS on α-amylase and α-glucosidase inhibition, however the effect of OS on pancreatic lipase inhibition and kinetics of α-amylase, α-glucosidase and pancreatic lipase inhibition were not studied. This study determined the in vitro inhibitory effects of OS against pancreatic lipase and characterized the kinetics of α-amylase, α-glucosidase and pancreatic lipase inhibition.
Materials and Methods: Dried powdered roots were successively extracted with various solvents such as petroleum ether, chloroform, ethyl acetate, and 70% ethanol. The successive extracts obtained (62.5-1000 μg/ml) were subjected to in vitro.. View More»
