Journal of Blood Disorders & Transfusion

Prabhu Manivannan

Publications

• Research Article
  Applicability of a Single 5 Color Cytoplasmic Markers Tube as Primary Panel in Routine Immunophenotyping of Acute Leukemia
  Author(s): Rahul Kumar Sharma, Vandana Puri, Deepti Mutreja, Sunil Kumar, Pratibha Chauhan, Abhishek Purohit, Prabhu Manivannan, Sudha Sazawal, Pravas Mishra and Renu SaxenaRahul Kumar Sharma, Vandana Puri, Deepti Mutreja, Sunil Kumar, Pratibha Chauhan, Abhishek Purohit, Prabhu Manivannan, Sudha Sazawal, Pravas Mishra and Renu Saxena
  
  Background: Immunophenotyping in acute leukemia is a routine practice for lineage assignment. Conventionally a primary panel with surface markers are applied first followed by cytoplasmic markers as secondary panel in the diagnosis of acute leukemia.We in this present study aim to assess the relevance of a single 5 color “CD45, MPO, CD79a, CD3, Tdt” cytoplasmic markers combination to be utilized as primary panel. Methods: Sensitivity and specificity of different subset of positive negative combination of these markers were retrospectively analyzed in the 458 acute leukemia cases. Results: MPO or cCD3 positivity alongwith cCD79a negativity was 100% specific diagnosis for AML and T-ALL respectively. Furthermore, cCD79a positivity along with MPO and cCD3 negativity was 97.2% specific for B-ALL diagnosis. MPO and cCD79a dual positivity was found 100% sensitive .. View More»
  DOI: 10.4172/2155-9864.1000309

  Abstract PDF