Journal of Clinical & Experimental Pharmacology

Peter J Little

Publications

• Research Article
  Methotrexate Inhibits Proliferation but not Proteoglycan Synthesis or Glycosaminoglycan Hyperelongation in Human Vascular Smooth Muscle Cells
  Author(s): Peter J Little, Robel Getachew, Danielle Kamato, Muhamad Ashraf Rostam, Neale Cohen, Vincent Chan and Narin OsmanPeter J Little, Robel Getachew, Danielle Kamato, Muhamad Ashraf Rostam, Neale Cohen, Vincent Chan and Narin Osman
  
  Objectives: Atherosclerosis is a disease process involving the early deposition of lipids in the vessel wall trapped by modified proteoglycans and subsequently a chronic inflammatory process leading to the clinical events. MTX has been chosen to study the potential efficacy of an anti inflammatory agent in preventing atherosclerosis and secondary cardiovascular disease in the cardiovascular inflammation reduction trial (CIRT). Methods: We have investigated cell proliferation and growth factor stimulated proteoglycan synthesis in vascular smooth muscle (VSMC) to assess some of the direct effects of MTX. Experiments were conducted in cultured human VSMC. Proliferation was assessed by the gold standard technique of cell counting and proteoglycan synthesis by 35S radiosulafate incorporation and size analysis by SDS PAGE. Key findings.. View More»
  DOI: 10.4172/2161-1459.1000181

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