Biochemistry & Analytical Biochemistry

Olga V Akopova

Publications

• Research Article
  The Use of Dichlorofluorescein as a Probe for Monitoring the Effects of Calcium on ROS Production in Mitochondria
  Author(s): Olga V Akopova, Liudmila Kolchinskaya and Valentina NosarOlga V Akopova, Liudmila Kolchinskaya and Valentina Nosar
  
  Dichlorofluorescein (DCF) is widely applied for monitoring reactive oxygen species (ROS) production in cells and isolated mitochondria. Our purpose was to compare different approaches to the use of DCF for monitoring ROS formation accompanying Ca2+ transport in isolated mitochondria. Ca2+ effects on ROS production was studied in rat brain and liver mitochondria using two modes of DCF application: the first was preloading of stock mitochondrial suspension with DCF precursor dichlorofluorescein diacetate (DCFDA), and the other was the addition of the aliquots of DCFDA directly to the incubation medium. Calcium increased ROS production in brain and liver mitochondria both under steady-state and non-equilibrium conditions caused by the gradual release of cytochrome c and the gating of the electron flow. Using DCF-preloaded mitochondria we have shown that the rate of ROS formation under st.. View More»
  DOI: 10.4172/2161-1009.1000334

  Abstract PDF