Neeraj Gilhotra
Rohtak – 124 001, Haryana
India
Research Article
Ethological Characterization and Pharmacological Validation of Elevated “I–maze” as Animal Model of Anxiety
Author(s): Ritu Gilhotra and Neeraj GilhotraRitu Gilhotra and Neeraj Gilhotra
Abstract Objective: The objective of the present study was to design a novel animal maze which can detect anxiety in mice and effect of different anxiogenic and anxiolytic treatments. Methods: The maze was behaviorally validated by recording the behaviors of mice on the maze before and after administration of anxiolytic drug treatments like Diazepam (1 and 2 mg/kg), Gabapentin (10 and 20 mg/kg), Fluoxetine (5 and 10 mg/kg), Ondansetron (0.1 and 1 mg/kg) and anxiogenic treatments like caffeine (15 and 30 mg/kg) and exposure to immobilization stress. Ethological characterization was done by tracking behavioral pattern of mice on the maze. Results: Diazepam significantly increased the percentage of time spent in the open areas (%TO) and the number of unprotected head dips (uHDIPS), and reduced the number of protected head dips (pHDIPS) and stretch attend postures (SAP) from close to open.. View More»
DOI:
10.4172/2161-1459.1000186