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Nasstasja Wassilew
Nasstasja Wassilew
Medical Department, Clinical Infectious Diseases, Research Center Borstel, Borstel,
Germany
Publications
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Research
Evaluation of the GenoType® NTM DR for Subspecies Identification and Determination of Drug Resistance in Clinical M. abscessus Isolates
Author(s): Nasstasja Wassilew, Doris Hillemann, Florian P. Maurer, Thomas A. Kohl, Matthias Merker, Folke Brinkmann, Marc Lipman and Katharina KranzerNasstasja Wassilew, Doris Hillemann, Florian P. Maurer, Thomas A. Kohl, Matthias Merker, Folke Brinkmann, Marc Lipman and Katharina Kranzer
Introduction: A new line probe assay, the GenoType® NTM DR, has been developed for subspecies identification and detection of resistance to macrolides and aminoglycosides in clinical Mycobacterium abscessus isolates. We studied the performance of the test compared to DNA sequencing and phenotypic drug susceptibility testing (pDST). Methods: 48 clinical M. abscessus isolates collected between 2015 and 2016 were identified to the subspecies level and analysed for erm (41) genotype, rrl and rrs gene mutations by Sanger sequencing. Broth micro dilution was performed for pDST of Clarithromycin and Amikacin. The results were compared to those of the GenoType® NTM DR assay. Discordant results were further analysed by repeat pDST and whole genome sequencing (WGS). Results: 35 isolates were identified as M. a.. View More»
DOI: 10.4172/2327-5073.1000286