Mary Ann Lila
Tanzania
Research Article
Lower Concentrations of Blueberry Polyphenolic-Rich Extract Differentially Alter HepG2 Cell Proliferation and Expression of Genes Related to Cell-Cycle, Oxidation and Epigenetic Machinery
Author(s): Farideh Shafiee-Kermani, Michael A Grusak, Sally J Gustafson, Mary Ann Lila and Mihai D NiculescuFarideh Shafiee-Kermani, Michael A Grusak, Sally J Gustafson, Mary Ann Lila and Mihai D Niculescu
In vitro cancer models have been used to study the effect of relatively high concentrations (>200 μg/ml) of phenolic plant extracts upon cell proliferation. In this study we report that the treatment of human hepatocarcinoma, HepG2, cells with lower concentrations of blueberry phenolic extract (6.5-100 μg/mL) for 96 h induced a non-linear response in cell proliferation, with a significant peak at 25 μg/mL and lower proliferation observed at higher concentrations, while no differences in apoptosis were present across groups. Flow cytometry analysis indicated a reduction of almost 19% of cells in S-phase for 25 μg/mL, as compared to control, while, no changes were observed for other concentrations. The percent of cells in G2/M phase was reduced at 50 μg/ml, while all other concentrations increased the percent of cells in G0/G1 phase... View More»
DOI:
10.4172/2161-0509.1000120