Clinical Microbiology: Open Access

Madhusudan Choudhary

Publications

• Research Article
  Identification and Characterization of Replication Origins of Multiple Chromosomes in Rhodobacter sphaeroides
  Author(s): Berra Koskulu, Abha Choudhary, Hannah Johnson, Hyuk Cho and Madhusudan ChoudharyBerra Koskulu, Abha Choudhary, Hannah Johnson, Hyuk Cho and Madhusudan Choudhary
  
  DNA replication has been extensively studied in a number of bacterial species, which possess a unipartite genome consisting of a single circular chromosome. Approximately 10% of sequenced bacterial species have a multipartite genome structure, which is comprised of multiple chromosomes. However, the coordination and regulation of multi-chromosomal replication in bacteria remains poorly understood. Rhodobacter sphaeroides possesses a multipartite genome consisting of two chromosomes, the primary chromosome (CI) of approximately 3Mb and the secondary chromosome (CII) of 0.9 Mb. Z-curve and GC skew analyses revealed that CI and CII of R. sphaeroides exhibit three and five putative chromosomal origin regions, respectively. Then, the flanking regions of these putative regions were further analyzed in terms of gene conservation, gene density, and gene ratios between the corresponding forwar.. View More»
  DOI: 10.4172/2327-5073.1000275

  Abstract PDF