Madan M Godbole
India
Short Communication
Wilms’ Tumor-1 (WT1) Protein in Urinary Exosomes Predicts Risk of Developing Proteinuria in Type-1 Diabetes
Author(s): Aradhana Mohan, Aditya Upadhyay, Madan M Godbole, Eesh Bhatia and Swasti TiwariAradhana Mohan, Aditya Upadhyay, Madan M Godbole, Eesh Bhatia and Swasti Tiwari
Objective: Previously, we reported presence of Wilms’ tumor 1 (WT1) in urinary exosomes in ~50% diabetic (T1D) population without proteinuria as oppose to 100% in T1D population with proteinuria. Here, we investigated if T1D subjects with detectable WT1 in urinary exosomes (UE) progress to proteinuria faster in a 2 year follow-up study. Method: 52 T1D subjects without proteinuria (age < 60 years) were enrolled. Urine and blood samples were collected for analysis. WT1 immunoblotting was performed on UE isolated by ultracentifugation of urine samples. Urine microalbumin, serum creatinine and glycated haemoglobin (HbA1c) were analyzed. Results: WT1 protein was detected in UE of 28 out of 52 T1D subjects. The data from followed patients, at the end of two years of follow up, revealed that 4 subjects (out of total 19) progressed to proteinuria with urine microalbumin >20 μg/.. View More»
