Luc Demaison
France
Research Article
Increased Abdominal Adiposity Exacerbates Ex-Vivo Cardiac Reperfusion Injury through Augmented Mitochondrial Oxidative Stress
Author(s): Damien Vitiello, Evangelia Mourmoura, Karine Couturier, Patrick Faure, Herve Dubouchaud, Corinne Malpuech-Brugere, Kasra Azarnoush and Luc DemaisonDamien Vitiello, Evangelia Mourmoura, Karine Couturier, Patrick Faure, Herve Dubouchaud, Corinne Malpuech-Brugere, Kasra Azarnoush and Luc Demaison
Compared to the lean Lou/C rat, the Wistar rat develops increased
body weight
and abdominal adiposity (IAA). The aim of this study was to compare the functional response to cardiac ischemia/reperfusion of 3-month old Wistar rats with age-matched Lou/C rats, and to explain the differences with regards to mitochondrial hydrogen-peroxide release (mH2O2r). Langendorff-perfused hearts of Lou/C and Wistar rats were subjected to ischemia (25 min) followed by reperfusion (30 min). Cardiac function was monitored throughout the experiment. Mitochondria were extracted before and after ischemia, and their oxidative capacities, mH2O2r, and activity of the respiratory-chain complex were measured. The IAA of Wistar rats was associated with slight glucose intolerance and noticeable functional abnormalities of the myocardium during post-ischemic reperfusion. Cardio-toxicity was rel.. View More»
