Clinical Microbiology: Open Access

Lise Hannecke

Publications

• Research Article
  16S-23S Intergenic Spacer (ITS) Region Sequence Analysis: Applicability and Usefulness in Identifying Genera and Species Resembling Non-Hemolytic Streptococci
  Author(s): Xiaohui Chen Nielsen, Derya Carkaci, Rimtas Dargis, Lise Hannecke, Ulrik Stenz Justesen, Michael Kemp, Jens Jørgen Christensen and Monja HammerXiaohui Chen Nielsen, Derya Carkaci, Rimtas Dargis, Lise Hannecke, Ulrik Stenz Justesen, Michael Kemp, Jens Jørgen Christensen and Monja Hammer
  
  The species that are catalase-negative, gram-positive cocci and not belonging to streptococci or enterococci have become increasingly well characterized and the number of taxonomic entities steadily growing based on molecular taxonomic studies. This complicates their identification. 16S-23S Intergenic Spacer (ITS) region sequence analysis has proven to be a useful tool for species identification of the genera Streptococcus and Enterococcus. This study investigated the possibility of using ITS sequence analysis as a common tool for species identification within the genera Aerococcus, Abiotrophia, Alloiococcus, Dolosicoccus, Dolosigranulum, Facklamia, Granulicatella, Gemella, Ignavigranum, Leuconostoc, and Vagococcus. ITS sequences of 29 type strains and 103 well-characterized clinical strains were determined and BLAST analysis performed for species identification. All clinical strains .. View More»
  DOI: 10.4172/2327-5073.1000130

  Abstract PDF