Biology and Medicine

Krassimir Yanev

Publications

• Research Article
  Investigation of Noninvasive Urine Specimens with Molecular Fluctuations for a Presence of High-Risk Human Papilloma Viruses as an Inflammatory Cofactor in the Prostate Cancer
  Author(s): Anita Ognyanova Kavrakova, Bilyana Georgieva, Kamen Anachkov, Krassimir Yanev, Georgi Ivanov, Vanyo Mitev and Albena TodorovaAnita Ognyanova Kavrakova, Bilyana Georgieva, Kamen Anachkov, Krassimir Yanev, Georgi Ivanov, Vanyo Mitev and Albena Todorova
  
  Background: The aim of the study is to investigate noninvasive urine specimens in suspected prostate cancer (PCa) patients by a panel of PCA3, TMPRSS2-ERG fusions and GSTP1 promoter hypermethylation. Furthermore, we tested urine specimens for the presence of high-risk Human Papilloma Viruses (HPV) as an inflammatory cofactor in the complicated origin of prostate cancer (PCa). Methods: A total of 50 patients with elevated PSA and/or PCa physiological symptoms were analyzed. RNA and DNA isolation; Reverse transcription; Real-time PCR; DNA sequencing; Bisulfite conversion of DNA; Methylationspecific PCR; Cytological preparations and staining were applied. Results: Molecular fluctuations were registered in most of the patients: neoplastic GSTP1 allele, PCA3 strongly elevated expression or hyperexpression. Only in 4 cases a positive TMPRSS2-ERG status was detected. High-risk HPV type.. View More»
  DOI: 10.4172/0974-8369.1000438

  Abstract PDF