Koldijk M
England
Research Article
H5N1 Protection by Seasonal Influenza Vaccine in Homologous and
Heterologous Prime/Boost Vaccination
Author(s): Roos A, Roozendaal R, Damman-Riahi S, Vreugdenhil J, Vaneman J, Dekking L, Koldijk M, Goudsmit J and Radošević KRoos A, Roozendaal R, Damman-Riahi S, Vreugdenhil J, Vaneman J, Dekking L, Koldijk M, Goudsmit J and Radošević K
Background
A number of different approaches aimed at broadening the cross-protective ability of seasonal influenza vaccines are being explored today. Priming a seasonal vaccine with three administrations of DNA encoding H1 HA corresponding to the HA in the seasonal vaccine has been shown to confer protection against heterologous H1N1 influenza. Here we evaluated the heterosubtypic protection induced by a seasonal influenza vaccine when primed with H1 HA DNA and, in parallel, when given as a homologous prime/boost regimen.
Methods
Balb/c mice were immunized three times with vaccine homologous H1 HA DNA prior to a boost with seasonal influenza vaccine (season 2009/2010; Northern Hemisphere), or immunized three times with the seasonal influenza vaccine. To assess cross-protection, mice were subsequently challenged with either heterologous H1N1 or heterosubtypic H5N1 .. View More»
DOI:
10.4172/2157-7560.1000299