Modern Chemistry & Applications

Khalid Muhammed Khan

Publications

• Research Article
  Short and Robust HPLC-UV Method to Determine Serum Ribavirin Concentration without Evaporation Step
  Author(s): Abdul Rafiq Khan, Ali Al-Othaim, Khalid Muhammed Khan, Shazia Mrtaza, Sara Altraif, Waleed Tamimi, Waqas Jamil and Ibrahim AltraifAbdul Rafiq Khan, Ali Al-Othaim, Khalid Muhammed Khan, Shazia Mrtaza, Sara Altraif, Waleed Tamimi, Waqas Jamil and Ibrahim Altraif
  
  Objective: Measurement of ribavirin (RBV) is important for therapeutic drug monitoring in hepatitis C patient. A simple and fast high performance liquid chromatography (HPLC) method developed and validated to measure ribavirin concentration in serum samples without an evaporation step. Design and method: About 500 μl serum sample, 50 μl internal standard and 20 mM ammonium acetate buffer (pH=8.5) were mixed for 30 seconds and centrifuged. The supernatant was transferred into preconditioned phenyl boronic acid cartridges for solid phase extraction. All cartridges were washed two times with 1 mL of 20 mM ammonium acetate buffer under vacuum not exceeding 10 psi. Ribavirin and internal standard were eluted with 300 μl of 3% formic acid. An aliquot of 100 μl was injected into HPLC system. Results: The metho.. View More»
  DOI: 10.4172/2329-6798.1000187

  Abstract PDF