Kenji Ohyama
Interdisciplinary Graduate School of Medicine and Engineering,
1110 Shimokato, Chuo-city, Yamanashi 409-3898
Japan
Research Article
Stage-Specific Expression Profiles of Anti-Müllerian Hormone and its Type II
Receptor in Germ Cells during Spermatogenic Cycle of Rats
Author(s): Kenji Ohyama, Masanori Ohta, Yoshinao Z Hosaka, Tetsuji Ohyama and Yoshiaki YamanoKenji Ohyama, Masanori Ohta, Yoshinao Z Hosaka, Tetsuji Ohyama and Yoshiaki Yamano
Although anti-Müllerian hormone (AMH) is present in seminiferous tubules, its physiological roles in testis remain unclear. We defined expression patterns of AMH and its type II receptor (AMHR2) in germ cells during spermatogenic cycles. To this end, we performed in situ hybridization of Amh and Amhr2, and immunohistochemical staining using antibodies against AMH and AMHR2 in testes from rats aged 10, 15, 21, 35, and 49 days (d). In addition, quantitative RT-PCR analyses were performed to determine relative expression levels of Amh, Amhr2, synaptonemal complex protein 1 (Scp1) and round spermatid basic protein 1 (Rsbn1), and Smads 1, 5, and 8 in germ cells isolated from 49d testes. In 49d testes, synthesis of AMH and AMHR2 corresponded with spermatocyte expression of Amh and Amhr2. Spermatocytes of stages VII-XIV synthesized AMH, and AMH staining was decreased in ro.. View More»
