Journal of Vaccines & Vaccination
Open Access
ISSN: 2157-7560
ISSN: 2157-7560
Keita Oma
Japan
Research Article
Comparative Effects of Toll-Like Receptor Agonists on a Low Dose PspA Intranasal Vaccine against Fatal Pneumococcal Pneumonia in Mice
Author(s): Zhenyu Piao, Keita Oma, Hirokazu Ezoe, Yukihiro Akeda, Kazunori Tomono and Kazunori OishiZhenyu Piao, Keita Oma, Hirokazu Ezoe, Yukihiro Akeda, Kazunori Tomono and Kazunori Oishi
To develop a cost-effective pneumococcal vaccine, we compared the effects of a panel of Toll-like receptor (TLR) agonists on a low dose pneumococcal surface protein A (PspA) nasal vaccine in a fatal pneumococcal pneumonia model using a serotype 3 strain. The mice were nasally immunized with 10 µg of the TLR agonist (TLR 2, 3, 4 and 9) and 0.1µg of PspA once per week for three weeks. A high level of PspA-specific immunoglobulin G (IgG) was detected in sera of mice that were nasally administered a low dose of PspA plus each TLR agonist, while no PspAspecific IgG were detected in sera of mice that had been nasally administered a low dose of PspA. A relatively low level of PspA-specific IgG was also detected in the airway of mice that had been nasally administered a low dose of PspA plus each TLR agonist. The binding of PspA-specific IgG increased the deposition of C3 on the b.. Read More»
DOI:
10.4172/2157-7560.1000113