John S Penn
Vanderbilt University School of Medicine,
8009 Medical Center East, 1215 21st Avenue South, Nashville, TN 37232-8808
Tanzania
Research Article
Linoleic Acid is a Diabetes-relevant Stimulator of Retinal Inflammation in Human Retinal Muller Cells and Microvascular Endothelial Cells
Author(s): Megan E Capozzi, Gary W McCollum, David B Cousins and John S PennMegan E Capozzi, Gary W McCollum, David B Cousins and John S Penn
Objective: To determine the effect of oleic acid and linoleic acid on the production and secretion of specific diabetic retinopathy- (DR-) related cytokines: vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), and interleukin-8 (IL-8) by human retinal glial cells, retinal endothelial cells, and retinal pigment epithelial cells. These expression profiles will be compared to those obtained by treatment of the same cell types with elevated D-glucose, a diabetes-relevant stimulus often used in retinal cell culture experiments.
Methods: Primary cultures of human retinal Müller cells, astrocytes, and microvascular endothelial cells (RMEC) and a human retinal pigment epithelial cell line (ARPE-19) were treated with oleic acid, linoleic acid, elevated Dglucose, or L-glucose as an osmotic control. VEGF, IL-6, and IL-8 .. View More»
