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Jasmani S
Jasmani S
Malaysia
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Research Article
Molecular Characteristic of Giant Grouper (Epinephelus Lanceolatus)Vitellogenin
Author(s): Om AD, Sharif S, Jasmani S, Sung YY and Bolong AAOm AD, Sharif S, Jasmani S, Sung YY and Bolong AA
The Vitellogenin (Vtg) gene sequence acts as an indicator to the fish reproduction, which can adapt to the environment factor or can influence the gonad development. The Vtg nucleotide sequence from Giant grouper was characterized using bioinformatics software. A homology search of the deduced amino acid sequence of the obtained Vtg DNAs (compare with 13 species) was carried out using the National Centre for Biotechnology Information website. Clustal W analysis was constructed a phylogenetic tree by using Molecular Evolutionary Genetic Analysis MEGA version 5.2.2. In order to verify the Vtg gene sequences obtaind and elucidate structure-function relationship in Vtg, by using DELTA BLAST of 3-D structure of Giant grouper with others fishes. Result of phylogenetic analysis using Maximum Likelihood (ML) and Neighbour Joining (NJ) showed tree analysis generated two separated tree topology.. View More»
DOI: 10.4172/2155-9546.1000360