Journal of Bacteriology & Parasitology

Humphrey Chukwuemeka Nzelibe

Publications

• Research Article
  Molecular Characterization of Enterotoxigenic Escherichia coli: Effect on Intestinal Nitric Oxide in Diarrheal Disease
  Author(s): Muhammad Arfat Yameen, Ebuka Elijah David, Humphrey Chukwuemeka Nzelibe, Muhammad Nasir Shuaibu, Rabiu Abdussalam Magaji, Amakaeze Jude Odugu and Ogamdi Sunday OnweMuhammad Arfat Yameen, Ebuka Elijah David, Humphrey Chukwuemeka Nzelibe, Muhammad Nasir Shuaibu, Rabiu Abdussalam Magaji, Amakaeze Jude Odugu and Ogamdi Sunday Onwe
  
  This study was aimed to investigate the effect of enterotoxigenic E. coli (ETEC)-induced diarrhea on fecal nitric oxide (NO) and intestinal inducible nitric oxide synthase (iNOS) expression in rats. E. coli isolates were gotten from infants diarrheal samples. Multiplex polymerase chain reaction (PCR) was used to detect the presence of eltA gene for heat-stable (ST) enterotoxigenic E. coli and eltB for heat-labile (LT) enterotoxigenic E. coli. Disk diffusion method was used to determine the antibiotic susceptibility of the organisms. Biofilm formation was detected by thiazoylblue tetrazolium bromide dye in a 96-well plate. Fecal NO was measured using standard griess reaction system. Reverse transcription PCR was used to investigate the expression of iNOS expression. Although none of the ETECs isolated in this study belonged to the c.. View More»
  DOI: 10.4172/2155-9597.1000339

  Abstract PDF